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Figure 9. The head and trunk sub-cluster genes show distinct responses to organizer signaling. Three genes were selected from each sub-cluster, and tested by in situ hybridization at stage 10.5 (top row) and 11.5 (bottom row), in embryos ventrally injected with noggin or noggin+dkk-1. Black arrowheads mark ectopic staining. Xlim-1 (A-F) and otx2 (M-R) expression are similarly induced at stage 10.5 by Noggin and Noggin+Dkk-1, but by stage 11.5 Noggin+Dkk-1 induction is clearly much stronger and more widespread. For Xlim-1, ectopic expression induced by Noggin+Dkk-1 is observed migrating away from the blastopore lip region, but never for Noggin alone. (M-R) Frzb-1 expression is ectopically induced only by the combination of Noggin+Dkk-1, not Noggin alone, and neither can sustain expression into late gastrulation. For the three trunk genes, FoxD5b (XFD-12') (S-X), FoxA4a (pintallavis) (Y-D'), and Xsox-2 (E'-J') ectopic induction is similar in both intensity and spread in Noggin and Noggin+Dkk-1 overexpressing embryos at stage 10.5 and 11.5. A-L and S-J' vegetal view; M-R animal view. Dorsal faces up in all pictures. (K') Otx2 expression was assayed by real-time RT-PCR, in stage 11.5 ventral tissues injected with the same mixtures used to create the microarray samples. Weak, but significant (p = 0.043 by one-sided t-test), induction in Dkk-1 overexpressing tissues are seen compared to ventral, supporting the weak Dkk-1 inductions seen on the microarray. Error bars show the standard error calculated from two biological replicates. Image published in: Hufton AL et al. (2006) Copyright © 2006 Hufton et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 10.5 involuting marginal zone upper blastopore lip dorsal marginal zone lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 11.5 involuted dorsal mesoderm upper blastopore lip otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 10.5 dorsal marginal zone otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 11.5 dorsal marginal zone frzb.S fiz, fre, frp-3, Frzb-1, frzb-pen, frzb1, fzrb, os1, sfrp3, srfp3, xfrzb X. laevis Throughout NF stage 10.5 upper blastopore lip frzb.S fiz, fre, frp-3, Frzb-1, frzb-pen, frzb1, fzrb, os1, sfrp3, srfp3, xfrzb X. laevis Throughout NF stage 11.5 involuted dorsal mesoderm foxd4l1.1.S foxd4, Foxd4l1, foxd4l1.1-a, foxd4l1.1-b, foxd5, foxD5a, FoxD5b, Xfd-12, XFlip, xlFoxD5a, xlFoxD5b, xtFoxD5 X. laevis Throughout NF stage 10.5 dorsal marginal zone foxd4l1.1.S foxd4, Foxd4l1, foxd4l1.1-a, foxd4l1.1-b, foxd5, foxD5a, FoxD5b, Xfd-12, XFlip, xlFoxD5a, xlFoxD5b, xtFoxD5 X. laevis Throughout NF stage 11.5 involuted dorsal mesoderm dorsal marginal zone foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Throughout NF stage 10.5 involuting marginal zone foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Throughout NF stage 11.5 involuted dorsal mesoderm sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 10.5 ventro-lateral marginal zone dorso-lateral marginal zone dorsal marginal zone sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 11.5 ventral marginal zone ventro-lateral marginal zone dorso-lateral marginal zone dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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