Xenbase Image ID: 76912

Additional file 3. St. 22 Hex-expressing anterior ventral endoderm is fated to give rise to liver precursors. A: st. 22 embryo, hybridised with Hex probe, is aligned along a graticule. B: strategy used for fate mapping the st. 224 Hex expressing ventral endoderm domain. In brief, embryos were aligned along the graticule using the pharyngeal arches, cement gland and a ventral protrusion as landmarks. The embryo was bisected caudal to the pharyngeal arches and the ventral protrusion and a small spot of DiI (Sigma) injected onto the exposed ventral endoderm. Bisected embryos were then grown until they reached st. 35. In situ hybridisation was performed to ascertain whether the position of the DiI corresponded to the position of Hexexpression. C, D: visible and fluorescent light views of st.35 half-embryos. E: merged images C, D. F: in s Image published in: Haworth KE et al. (2008) Copyright © 2008 Haworth et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue hhex.L hex, tHex, XHex X. laevis Throughout NF stage 22 liver primordium Image source: Published Larger Image Printer Friendly View

Return to previous page