XB-IMG-76914
Xenbase Image ID: 76914
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Figure 6. Injection of GATA4 and GATA5 MOs in endoderm interferes with heart morphogenesis. Top: experimental scheme showing targeted co-injections of MOs and rhodamine-dextran (red fluorescence). The drawing of ventral view of Nieuwkoop and Faber stage 41 embryo [55] is from http://www.xenbase.org webcite. Cardiac actin/GFP embryos were injected at 32-cell stage with indicated MOs and the fate of injected cells was recorded at st. 41 by rhodamine fluorescence (B, D, F, H, J). A, C, E, G, I: GFP fluorescence was used to observe heart development (cardiac actin/GFP is expressed in all striated muscles, and head muscles are visible in all images), and GFP images were merged with rhodamine images to highlight injected areas. Heart is indicated by arrows, and in specimens where heart was partially injected (C, G), uninjected area is highlighted by an arrowhead. All properly targeted embryos were similarly affected by G4SP and G5SP MOs (n = 8, 10, respectively), but not by C1 MO (n = 7). Ventral views are shown, with anterior at the top. Image published in: Haworth KE et al. (2008) Copyright © 2008 Haworth et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |