Xenbase Image ID: 76933
Additional file 4. The jovan heat (joh) allele has a Tol2XIG transposon integrated near the HNF1β gene (a) Schematic representation of the Tol2XIG integration event in joh (not to scale). The transposition reaction resulted in integration of the transposon in intron 9 of a novel HEAT motif-containing gene on scaffold 512:565147. In situ hybridization with antisense RNA probes generated to the HEAT repeat cDNA indicated low-level ubiquitous expression of the HEAT motif-containing gene that lacked robust expression in the developing kidney (data not shown). The HNF1β gene flanks the 3' end of the HEAT motif-containing gene and is approximately 46 kb from the Tol2XIG transposon. (b) In situ hybridization for HNF1β expression during Xenopus development shows intense staining in the developing kidney . Antisense RNA probes were synthesized from a Xenopus laevis HNF1β cDNA (IMAGE 4959359). In situ stained albino Xenopus laevis embryos shown at stages 27 and 35 (anterior is facing to the right, dorsal up). Arrows point to the developing kidney.
Image published in: Yergeau DA et al. (2010)
Image downloaded from an Open Access article in PubMed Central. Copyright ©2010 Yergeau et al; licensee BioMed Central Ltd.
Image source: Published
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