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Fig. S3. Confirmation of the morpholino specificity. A tracer, Alexa 594-dextran (300 pg), GFP constructs, and morpholinos were injected in the animal pole of four-cell embryos and were observed at stage 10 to show the injected embryos (Top Row), the fluorescent Alexa 594-dextran tracer (Middle Row), and GFP fluorescence (Bottom Row). Xfurry-MO specifically reduced translation of sequences containing Xfurry-GFP targeted with Xfurry-MO at the 5′-region but did not reduce translation of 5-mis-Xfurry-GFP, which has no targeted sequence.

Image published in: Goto T et al. (2010)

Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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