Xenbase Image ID: 76967

Fig. S4. Rescue experiments among Xfurry, Xfurry-MO, enR+LZ, and VP16+LZ. (A) Control (stage 33). (B) Injection of Xfurry-MO (5 ng/blastomere) into dorsal blastomeres of four-cell embryos interfered with head formation and shortened the axis of all embryos, as shown also in Fig. 1K (n = 40). (C) The cement gland was rescued, and the axis was slightly elongated by coinjection of Xfurry (2 ng/blastomere) and Xfurry-MO (5 ng/blastomere) into dorsal blastomeres of fourcell embryos (57.9%; n = 57). (D) Dorsal expression of enR+LZ (400 pg/blastomere) also rescued the phenotype of Xfurry-MOnjected embryos (78.6%; n = 56). (E) Dorsal injection of VP16+LZ (400 pg/blastomere) interfered with head formation and shortened the axis of all embryos, as in Fig. 2G (n = 40). (F) Dorsal expression of Xfurry (2 ng/blastomere) rescued the phenotype of VP16+LZ-injected embryos (52.5%, n = 59). Image published in: Goto T et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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