Xenbase Image ID: 76977

Fig. 4. Fgf signalling is necessary for heart patterning and morphogenesis. Embryos were treated with SU5402 during different developmental windows to determine when Fgf signalling is necessary for heart development and assayed for expression of nkx2.5 (AD and IL) or tnni3 (EH and MP). Fgf signalling was inhibited at successively later stages (top panel: AH) and compared to control embryos (A and E). Embryos treated with SU5402 at either stage 12.5 (B and F) or stage 20 (C and G) demonstrate a complete loss of heart marker expression by stage 32, while embryos treated at stage 24 (D and H) show expression of both nkx2.5 and tnni3 but no discernable heart tube. Fgf signalling was also inhibited at stage 12.5 and restored at later stages by removing the inhibitor (bottom panel: IP) and compared to control embryos (I and M). When Fgf signalling is restored by either stage 20 (J and N) or stage 22 (K and O), both heart markers are expressed however a normal heart tube is not formed. If signalling is not restored until stage 26 (L and P), neither nkx2.5 or tnni3 are detectable. White arrows mark the heart region. The total number of embryos examined for each panel is indicated in the lower left hand corner. Image published in: Deimling SJ and Drysdale TA (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 32 heart tnni3.L c-troponin, cardiac tropin T, cardiac troponin I, cTnI, ctnIc, cTNT, TIc, TnIc, tnnic, troponin-ic, XcTnI, XTnIc X. laevis Throughout NF stage 32 heart Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + su5402 (@12.5) + NF32 (in situ hybridization) Fig. 4 (c2) Expression Phenotype absent nkx2-5.L expression in heart primordium absent tnni3.L expression in heart primordium Xla Wt + su5402 (@NF20) + NF32 (in situ hybridization) Fig. 4 (c3) Expression Phenotype absent nkx2-5.L expression in heart primordium absent tnni3.L expression in heart primordium Xla Wt + su5402 (@NF24) + NF32 (in situ hybridization) Anatomical Phenotype absent heart Expression Phenotype decreased amount nkx2-5.L expression in cardiac mesoderm decreased amount tnni3.L expression in cardiac mesoderm Larger Image Printer Friendly View

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