Xenbase Image ID: 76981

Fig. 8. Retinoic acid and fgf are opposing signalling molecules in patterning the LPM. Embryos were treated at stage 12 with either RA, SU5402 or both RA and SU5402 and assayed by whole mount in situ hybridization for expression of nkx2.5 (arrowhead a), isl1 (arrowhead b) and foxf1. Treating embryos with RA reduces the expression domain of both nkx2.5 (B) and isl1 (F) compared with controls (A and E). Significantly reduced domains of nkx2.5 and isl1 were also present in SU5402 treated embryos (C), however when embryos are treated with both RA and SU5402 neither marker is detectable (D and H). The expression domain of foxf1 was expanded in both the RA (J) and SU5402 (K) treatments when compared to controls (I), however when embryos were treated with both RA and SU5402, foxf1 expression was detectible across the entire LPM although expression was still graded. Ant: anterior view with dorsal at top of image. Pos: posterior view with dorsal at top of image. Llv is left lateral view. The total number of embryos examined for each panel is indicated in the lower left hand corner. Image published in: Deimling SJ and Drysdale TA (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 20 cardiac mesoderm isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 20 cardiac mesoderm neural crest foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Throughout NF stage 20 lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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