Xenbase Image ID: 76984

Supplementary Fig. 2. Altering the bio-availability of endogenous RA ligand by inhibiting members of the RA signalling pathway leads to similar effects as exogenous RA treatments. Expression of cyp26, a biomarker of RA signalling, is slightly increased under treatment with ketoconazole (B and E), and decreased with DEAB (C and F) as compared to control embryos (A and D). The anterior fgf8 expression domain (arrowhead a) was unchanged with a moderate increase in RA signalling when treated with ketoconazole (H) was also reduced in comparison to expression in the pituitary anlagen (arrowhead b) under treatment with DEAB (I) when compared to controls (G). However consistent changes were seen with either ketoconazole or DEAB on the posterior domain of fgf8 expression (J–L). Also, no obvious and consistent changes were seen on the size and position of the sprouty2 domain (M–R). Image published in: Deimling SJ and Drysdale TA (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue cyp26a1.L cyp26, RACE, retinoic acid converting enzyme, xCYP26, XCYP26A1 X. laevis Throughout NF stage 18 presumptive midbrain-hindbrain boundary anterior neural ridge paraxial mesoderm fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 18 preplacodal ectoderm anterior neural ridge presumptive midbrain-hindbrain boundary adenohypophyseal placode circumblastoporal collar spry2.S sprouty 2, sprouty-2, sprouty2, Xsprouty2, xspry2, Xtsprouty2 X. laevis Throughout NF stage 18 neural fold preplacodal ectoderm presumptive midbrain-hindbrain boundary anterior neural ridge Image source: Published Larger Image Printer Friendly View

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