Xenbase Image ID: 76985

Supplementary Fig. 3. ATP promotes the efficacy of SU5402. (A) Embryos were treated with DMSO + ATP, 10 μM SU5402 or 10 μM SU5402 in conjunction with 0.1 mM ATP. Embryos treated with SU5402 alone elongated similar to DMSO controls, while embryos treated with SU5402 in conjunction with ATP demonstrate the truncated phenotype characteristic of a loss of FGF signalling. (B) Similar results are obtained when using cardiac differentiation, as assayed by expression of tnni3, although lower concentrations of SU5402 were effective. When embryos were exposed to 1 μM SU5402 alone cardiac differentiation was similar to controls but when embryos were exposed to 1 μM SU5402 in conjunction with 0.1 mM ATP, cardiac differentiation was blocked. Image published in: Deimling SJ and Drysdale TA (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tnni3.L c-troponin, cardiac tropin T, cardiac troponin I, cTnI, ctnIc, cTNT, TIc, TnIc, tnnic, troponin-ic, XcTnI, XTnIc X. laevis Throughout NF stage 32 heart Image source: Published Larger Image Printer Friendly View

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