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Supplementary Fig. 4. Sprouty2 expression demonstrates the efficacy of SU5402. Treatment of embryos at stage 12.5 with 10 μM SU5402 with 0.1 mM ATP is sufficient to largely eliminate sprouty2 expression based on whole mount in situ hybridization when assayed at either stage 20 (A–D) or stage 32 (E F). Image published in: Deimling SJ and Drysdale TA (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
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