Xenbase Image ID: 77074

Figure 6. Eomes Can Induce Muscle and Notochord in Animal Caps, Activate gsc in the Ventral Equatorial Region, and Change Cell Fate(A and B) Sections through animal caps previously injected with 9 ng (A) or 18 ng (B) Eomes mRNA. Sections were stained with the 12/101 antibody for muscle (black) or with MZ15 antibody for notochord (red).(C) In situ hybridization to sections cut vertically through the dorsal-ventral axis. Embryos were injected in the ventral equatorial region at the four-cell stage with Eomes mRNA: 200 pg (C), 5 ng (D), or 18 ng (E and F). (F) shows an Eomes-induced secondary axis (arrows). As a control, Xbra mRNA was injected ventrally: 5 ng (G) or 18 ng (H). In situ hybridization to gsc transcripts (purple color) shows ectopic activation of gsc on the ventral side by Eomes, but not by Xbra. Dorsal (D) and ventral (V) sides, muscle (m), and notochord (n) are marked. Image published in: Ryan K et al. (1996) Copyright © 1996. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page