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Figure 7. Gastrulation Is Arrested and Axial Differentiation Inhibited by Reduction of Eomes Function(A), arrested midgastrula; (B), severe exogastrula (stage 37) after injection of 1 ng Eomes-Engrailed mRNA; and (C), normal stage 37 tadpoles after injection of 3 ng Engrailed mRNA. (D), RNase protection analysis of stage 37 embryos injected with 1 or 6 ng Eomes-EnR compared to whole embryos (WE). (E), first column, dissected dorsal embryo halves (stage 16) that were injected with 1 ng of Eomes-EnR at the 4-cell stage. Middle column, axial structures and MyoD expression are rescued in dorsal explants by coexpression of wild-type Eomes (4 ng) with Eomes-EnR (1 ng). Last column, Eomes alone (4 ng) does not block MyoD expression in dorsal explants. Top row (Whole) shows fixed dorsal explants, middle row shows MyoD stained sections, and bottom row shows Hoescht stained sections. Image published in: Ryan K et al. (1996) Copyright © 1996. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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