Xenbase Image ID: 77155

Fig. 1. vpp1 and hhex display partially overlapping expression in gas- trulating dorsal endoderm and later demarcate ventral pancreatic buds and liver anlagen, respectively. (A) Comparison of vpp1 and hhex expression as revealed by whole mount in situ hybridization analysis with bisected (stages 118) or whole (stages 243) embryos. (1, 2, 5, 6, 9, 10, 13, and 14) Dorsal is toward the right; (172) lateral view, head toward the left. Images 3, 7, 11, and 15 are cartoons drawn representing both the single staining (images 1, 2, 5, 6, 9, 10, 13, and 14) and double staining whole mount in situ hybrid- ization data (Fig. S3B), illustrating vpp1 (purple) and hhex (turquoise) ex- pression. Boxed regions are magnified in 4, 8, 12, and 16, respectively. (B) RT-PCR analysis compares the temporal expression profile of vpp1 and hhex during Xenopus embryogenesis. Ornithine decarboxylase (odc) was used as the RNA loading control. Image published in: Zhao H et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hhex.L hex, tHex, XHex X. laevis Throughout NF stage 11 endomesoderm involuted dorsal mesoderm hhex.L hex, tHex, XHex X. laevis Throughout NF stage 12 endomesoderm anterior gastrocoel roof plate hhex.L hex, tHex, XHex X. laevis Sometime during NF stage 13 to NF stage 18 anterior gastrocoel roof plate prechordal plate hhex.L hex, tHex, XHex X. laevis Throughout NF stage 24   hhex.L hex, tHex, XHex X. laevis Throughout NF stage 28   hhex.L hex, tHex, XHex X. laevis Throughout NF stage 33 and 34   Image source: Published Larger Image Printer Friendly View

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