XB-IMG-77158
Xenbase Image ID: 77158
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Fig. 4. Ectopic hhex-induced ventral pancreatic progenitors undergo normal endocrine and exocrine differentiation. Whole mount staining was performed with molecular probes indicated on the left. (A) Lateral view, head toward the left. Phenotype is as follows: B, 18/20; D, 40/40; F, 21/21; H, 31/31; J, 19/19; L, 32/32. (M and N) Right side views of the same embryos in K and L, respectively, showing that in the control embryo, because of the unilateral location of pancreas, stomach, and duodenum on the left side of the embryo at this developmental stage, fibrinogen stains a continuous area between the intestine and the liver bud. In contrast, in hhex-injected embryos, the anterior, fibrinogen-positive area is interrupted by the ectopically formed ventral pancreatic cells. (O and P) Ventral view, head toward the top. Phenotype is as follows: P, 51/51. (Q and R) Pancreata isolated from elastase: GFP transgenic Xenopus tadpoles (stage 47) with or without hhex over-expression. They were kept in the same Petri dish and photographed together in the same visual field and magnification. The dashed white line was added for a better alignment. Phenotype is as follows: R, 32/32. (S) Whole mount in situ hybridization of insulin on isolated liver and annular pancreata. Red asterisks highlight liver lobes. U shows the other side of the same liver and pancreata in T. The darker structure above the red asterisk in U is the deformed gall bladder. Note that there are two liver lobes in control embryos, but only one liver lobe in hhex-injected embryos (100%, n = 11). Image published in: Zhao H et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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