Xenbase Image ID: 77172

Fig. 1. Schematic of experimental strategy. (A) Xenopus embryos were injected into both animal blastomeres at the two-cell stage with Gata5 (500 pg) mRNA. Ectodermal explants were isolated at stage 9 from both Gata5-injected and uninjected embryos and cultured until stage 28 (tailbud). Transcriptional differences were analyzed on 5000-clone gastrula-stage cDNA microarray (Munoz-Sanjuan et al., 2002). An aliquot of each RNA sample was assayed for expression of the indicated Gata5 targets by RT-PCR. ODC was used as loading control. (B) Pie chart of the classification of the genes upregulated by Gata5 based on the GO molecular function categories. The majority of the upregulated clones in the Gata5 array fall into four main categories: (1) catalytic activity (26%); (2) binding activity (26%), including the large group of nucleic-acid-binding as well as protein-binding factors; (3) hypothetical/unknown function (13%), including full-length sequences conserved in the mouse and human databases the function of which is unknown; (4) no database match (13%), including a number of clones with no hits in database searches, which might be either genes unique to the frog or partial cDNAs. Edd, endodermin; -RT, minus reverse transcriptase; ODC, ornithine decarboxylase. Image published in: Spagnoli FM and Brivanlou AH (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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