XB-IMG-77207
Xenbase Image ID: 77207
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Fig. 1 – High potassium inhibits the induction of dendrites promoted by BMP-2. Dissociated sister retinal cultures from stage 24 embryos were treated either with control medium, 30 mM KCl, 50 ng/ml BMP-2 or 50 ng/ml BMP-2 + 30 mM KCl for two days. Fixed cultures were immunostained for the RGC specific axonal marker, neurofilament associated antigen. NAApositive axons are indicated by arrowheads. (A–H) Phase contrast photomicrographs of RGCs in several different treatments, with their respective NAA immunolabeling. (A and B) control. (C and D) 50 ng/ml BMP-2. Arrows point to the NAA-negative,
dendrite-like structure. (E and F) 30 mM KCl. (G and H) 50 ng/ml BMP-2 + 30 mM KCl. Scale bar in A is 10 lm for A–D and 20 lm for E and F. (I) The mean percentage of NAA positive RGC somata that had dendrite-like NAA negative processes is shown for each experimental condition. Data is the mean of three independent experiments. *p < 0.05, One way ANOVA, Dunnett’s post
hoc test. Image published in: Hocking JC et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |