XB-IMG-77208
Xenbase Image ID: 77208
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Fig. 2 – GFP and the transgenic kV channel proteins are colocalized within RGC dendrites. At stage 19, the developing neuroepithelium that gives rise to the eye was transfected with either WTKv1.1 (A–C) or DNKv1.1 channel cDNA (D–F)
constructs. Embryos were left to develop until stage 39/40,
then fixed and frozen transverse cryostat sections cut
through the central portion of the retina. Sections were
immunostained with antibodies against GFP and the myc
tag (9E10) of the kV channel transgenes. The GFP (B and E)
and myc immunoreactivity (A and D) were co-localized in
RGCs (merge shown in C and F), indicating that myc
immunoreactivity gives a similar representation of the
dendritic arbor as GFP. Scale bar in A is 15 lm. Image published in: Hocking JC et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |