XB-IMG-77209
Xenbase Image ID: 77209
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Fig. 3 – Misexpression of the WTKv1.1 potassium channel transgene enhances RGC dendrite growth. At stage 19, the developing neuroepithelium that gives rise to the eye was transfected with either GFP, WTKv1.1 or DNKv1.1 channel cDNA constructs. Embryos were left to develop until stage 39/40, then fixed and wholemount immunostained with antibodies
against either GFP, or myc for the kV channel constructs. RGCs were visualized in 50 lm vibratome sections. (A–D) Photomicrographs of representative RGCs expressing GFP (A), DNKv1.1 (B), or WTKv1.1 (C and D) are shown. When necessary, digital composites of different focal planes were assembled to represent the entire RGC dendritic arbor. Arrowheads mark the
RGC axons and arrows the dendrites. RGCL, retinal ganglion cell layer; IPL, inner plexiform layer; L, lens. Scale bar in A is 20 lm. (E–H) Graphs of different parameters of the dendritic arbor for GFP-, WTKv1.1- and DNKv1.1-expressing RGCs. Shown are the mean number of primary dendrites (E), the average length of individual primary dendrites (F), the mean number of branch points (G), and the mean total length of the branches (H). Data is from 5 independent sets of transfected embryos. Error
bars are s.e.m. *p < 0.05, repeated measures One Way Anova, Dunnett’s post hoc test. Image published in: Hocking JC et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |