Xenbase Image ID: 77210

Fig. 4 – Potassium channel constructs inhibit light evoked expression of the neural activity dependent marker c-fos. Embryos were electroporated at stage 28 with CS2-GFP alone (A, B, D and E), or CS2-GFP with either CS2-Kir2.1-myc (C, F) or CS2-WTKv1.1-myc. At stage 32 embryos were moved to the dark, and at stage 40/41 stimulated for 90 min with room light before fixation and processing for c-fos immunoreactivity. (A–F) Panels represent GFP fluorescence (A–C) and c-fos immunoreactivity (D–F) for dark-adapted CS2-GFP electroporated retinas exposed to light for 0 h (A and D) or 90 min (B and E), or dark-adapted CS2-GFP + CS2-Kir2.1-myc electroporated retinas exposed to light for 90 min (C and F). Asterisks label c-fos positive GFPexpressing cells in the RGC layer. Note that Xenopus embryonic tissue itself has some low level autofluorescence and yolk granules are sometimes evident (arrowhead). (G and H) Graphs showing the percentage of GFP-expressing cells in the RGC layer that are immunopositive for the immediate early gene protein c-fos. The data for CS2-Kir2.1 overexpression is shown in G, and for CS2-WTKv1.1 is shown in H. Numbers in brackets are the numbers of GFP-expressing cells. A chi-square statistical analysis was performed for the data in G (chi-square = 449.5, df 3) and H (chi-square = 50.3 df 3). Image published in: Hocking JC et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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