XB-IMG-77211
Xenbase Image ID: 77211
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Fig. 5. Misexpression of an inwared rectifier channel and inhibition of voltage-gated calcium channels promotes RGC
dendrite branching. (A–C) RGCs in stage 39/40 retinas electroporated at stage 28 with dnKv1.1-myc (A, A') or Kir2.1-myc (B and C, B' and C') cDNA constructs. Composites of photomicrographs taken at 1–3 different focal planes are shown in A-C, andcartoons of the corresponding cells shown in A'–C'. Myc immunolabeling is shown for the cells in A and C, and the inset forthe cell in B. B shows GFP fluorescence. (D) Graph showing the mean number of dendrite branch points. Numbers in brackets
are the numbers of cells, pooled from two independent experiments. Errors are s.e.m, ***p < 0.001, un-paired, two-tailedStudent’s t-test. (E and F) Retinas were electroporated at stage 28 with CS2-GFP. At stage 33/34, the skin around the eye was surgically removed, and embryos bathed either in control MBS solution, or in MBS containing 200 lM NiCl2 until stage 40.
GFP-expressing RGCs exposed to control (E) and NiCl2 (F) solutions. Photomicrographs (E-F) are composites of 1–3 different
focal planes, and corresponding schematic images (of the cells shown in E'–F'. Schematics were generated directly from tracing high magnification images of the RGCs. Arrows mark the axons, white arrowheads the dendrites, and black
arrowheads the dendrite branch points. (G) Graph showing the mean number of dendrite branch points for RGCs in control and NiCl2 conditions. Numbers in brackets are the numbers of cells, pooled from two independent experiments. Error bars are s.e.m.**, p < 0.01, un-paired, two-tailed student’s t-test. Scale bar in A is 10 lm. IPL, inner plexiform layer; L, lens. Image published in: Hocking JC et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |