Xenbase Image ID: 77224

Figure S1. Efficacy of antisense morpholinos used in this study. Stage 35 embryos injected with the indicated MOs reproduced published phenotypes. Standard control-MO injected embryos have no phenotype. B-catenin-MO results in ventralized embryos (Heasman et al., 2000). Co-injection of Sia-MO + Twn-MOs results in anterior truncation (Ishibashi et al., 2008). Injection of Tcf3-MO results in truncated heads and short axis (Liu et al., 2005). Co-Injection of Vent1-MO + Vent2-MO (Sander et al., 2007) results in loss of ventralosterior structures, while injection of Gsc-MO (Sander et al., 2007), Chordin-MOs (Oelgeschlager et al., 2003) plus Noggin-MO (Kuroda et al., 2004) and the Otx2-MO (Carron et al., 2005) result in loss of dorsalnterior structures. The Lim1-MO causes gastrulation defects (Schambony and Wedlich, 2007). Injection of Smad2a-MO + Smad2b-MO, which target both Xenopus laevis Smad2 pseudo alleles results in a phenotype similar to Nodal inhibition by cerb-S. Image published in: Rankin SA et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + chrd MO + nog MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormally posterioralized embryo abnormally ventralized embryo Xla Wt + ctnnb1 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormally ventralized embryo Xla Wt + gsc MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormal head morphology abnormal tail morphology Xla Wt + lhx1 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormal gastrulation abnormal head morphology abnormal tail morphology abnormal ventral trunk morphology Xla Wt + lhx1 MO + otx2 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormal embryo morphology abnormal gastrulation Xla Wt + otx2 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormal head morphology abnormal tail morphology Xla Wt + sia1 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormal tail morphology abnormally posterioralized embryo Xla Wt + smad2 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormally ventralized embryo Xla Wt + tcf7l1 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the head Xla Wt + ventx1.1 MO + ventx2.1 MO + NF33/34-35 and 36 (morphology) fig.S1 Anatomical Phenotype abnormally anterioralized embryo abnormally dorsalized embryo Larger Image Printer Friendly View

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