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Supplementary Fig. S1. Temporal and spatial gene expression patterns of VegT during oogenesis and embryogenesis of X. tropicalis. (A)
Temporal expression was analyzed by semi-quantitative RT-PCR. Primer pairs specific for X. tropicalis ODC were described previously (Haramoto et al., 2004). The primers designed for X. tropicalis mVegT are as follows: 5 ATGAGAAACTGCTGTCAGGAACAC-3and 5TGAAACCTGGGCTTGTAGCG- 3 (B-R) Spatial expression was analyzed by WISH. Roman figure shows stage of oogenesis.