Xenbase Image ID: 77241

Figure 4. Immunostaining of otic epithelia. (A, A', B, B', C, C') ISH for Tbx2 was performed at stage 26. Embryos were then sliced and immunostained for different epithelial markers, like (A, A') fibronectin (n = 23), (B, B') aPKC (n = 19) and (C, C') C-Cadherin (n = 23) in red. Nuclei were stained with DAPI in blue. The otocyst region could be identified in the sections by Tbx2 expression. (A', B', C') Injected otocysts show epithelial disorganization indicated through inaccurate nuclei alignment and displaced epithelial markers (arrows). (D, D') Rose diagram to highlight the orientation of cells in wildtype (D) compared to morphant (D') otocyst region. Wildtype otocysts show cells orientated mostly in angles between 0 and 45hile morphant otocysts display angles between 0 and 180 (E) Demonstration of the angle measurements: orientation of DAPI stained nuclei in relation to a horizontal median through the otocyst. Scale bar 20 μm. Image published in: Jung B et al. (2011) Copyright ©2011 Jung et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue fn1.S fibronectin, gene C, LOC121393057, MGC97508 X. laevis Throughout NF stage 26 otic placode epithelial cell prkci.L apkc, atypical protein kinase c, pkc-iota, PKCiota X. laevis Throughout NF stage 26 otic placode epithelial cell cdh3.S b-cadherin, c-cadherin, cdh3-a, cdh3-b, cdhc-A, cdhp, EP-cadherin, hjmd, P-cadherin, pcad, U-cadherin, xb-cad, XB-cadherin, XBcad X. laevis Throughout NF stage 26 otic placode epithelial cell Image source: Published Larger Image Printer Friendly View

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