Xenbase Image ID: 77260

Fig. 1. Cell proliferation in the stage 31 (A), 37 (B), 42 (C) and 45 (D) X. laevis inner ear, three hours after transient exposure to BrdU. Representative anterior to posterior sections (A1-A3; B1-B3; C1-C3; D1-D3) show BrdU-positive cells labelled with anti-BrdU antibody (red/brown; arrows) and counterstained with hematoxylin (blue/violet). (A) At stage 31, BrdU-positive cells are distributed throughout the epithelium of the otic vesicle (A1-A3). (B-D) Few BrdU-positive cells are detected in the emerging saccular epithelium (asterisks), while BrdU-positive cells can be seen flanking these regions in the stage 37 (B1-B3), 42 (C1-C3) and 45 (D1-D3) inner ear. BrdU-positive cells (arrows) can be seen restricted to other regions of the otic epithelium and within the sensory ganglion at stage 42 (C1-C3) and 45 (D1-D3). BrdU-positive cells (dashed circles) are visible in the ventricular zone of the brain in all stages (A4, B4, C4, D4). Control sections processed for immuno- histochemistry with secondary antibody but without primary (anti-BrdU) antibody and counterstained with hematoxylin (blue/violet) are shown in panels A5, C5, D5; panel B5 shows a control section that was not counterstained with hematoxylin. Scale bar, 100 μm. Image published in: Quick QA and Serrano EE (2007) Copyright © 2007. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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