Xenbase Image ID: 77307

Figure 5. CRIM1 is required for junctional localization of catenin in the neural plate. (A) Whole-mount immunofluorescence labeling of stage 13 Xenopus embryos in which standard control MO (A, C) or translation-blocking XLCAB MOs (B, D) were co-injected with dextran tracer at the 4-cell stage. Embryos were labeled with antibodies to C-cadherin and catenin as indicated. (C) Higher magnifications of the indicated regions of (A and B). (E) Pixel intensity histograms of imuunofluorescence labeling for the line intervals shown in (A and B) respectively. (G) Magnified regions corresponding to the line intervals shown in (A and B) with color channel merge (top) and separated color channels corresponding to different labels as indicated. (I) Quantification of relative expression levels of C-cadherin and catenin in standard control MO and XLCAB injected embryos by normalizing average pixel intensities over 150-pixel line intervals in tracer-positive regions to those of tracer-negative regions within the same embryo. In XLCAB injected embryos, relative expression levels were measured over intervals placed exclusively in tracer-positive, adherent regions or tracer positive, non-adherent regions (n = 20 in all categories) (J) Immunofluorescence labeling of cryosections from Xenopus embryos at stage 16 (mid-neurula) of mildly affected embryos. MOs were co-injected with dextran tracer (green). Cryosections were labeled with Hoechst 33258 for nuclei (blue) and with antibodies to catenin (J, K, white). Tracer-positive regions are outlined with a dashed white line. The gray line between panels indicates separated color channels of the same image. doi:10.1371/journal.pone.0032635.g005 Image published in: Ponferrada VG et al. (2012) Ponferrada et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue cdh3.S b-cadherin, c-cadherin, cdh3-a, cdh3-b, cdhc-A, cdhp, EP-cadherin, hjmd, P-cadherin, pcad, U-cadherin, xb-cad, XB-cadherin, XBcad X. laevis Throughout NF stage 13 neural plate ctnnb1.S B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 13 neural plate ctnnb1.S B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 16 neural plate neural fold Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + crim1 MO + NF13 (immunohistochemistry) Fig 5 B,D,F,H Expression Phenotype decreased amount cdh3.L expression in apical junction complex decreased amount ctnnb1.L expression in apical junction complex Larger Image Printer Friendly View

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