Xenbase Image ID: 77335

Fig. 2. Xenopus tadpoles transgenic for PDX-1-GFP. (a,b) GFP reporter activity in live tadpoles. Green fluorescence shows where the reporter is active, and yellow colour is due to yolk autofluorescence. (a) Three-day-old tadpole from left hand side showing GFP in the pancreatic and duodenal endoderm (black arrow) and lens of the eye (white arrow). Some of the melanocytes, which partially obscure the gut at this stage, have been removed. Anterior is to the left. (b) Four-day-old tadpole viewed from the right hand side to show GFP in the pancreas (pa) but not in the surrounding gut coils. Anterior is to the right. (c) In situ hybridisation to show GFP mRNA in pancreatic buds and duodenum of 3-day-old transgenics (dark blue). View from the left, melanocytes covering the gut have been removed to improve visibility. Anterior is to the left. (d) In situ hybridisation to detect GFP mRNA in whole guts taken from transgenic tadpoles. By 6 days, GFP expression is no longer visible in the pancreas (pa), but low levels of expression are maintained in the duodenum (du). Anterior is to the left. Abbreviations: pa, pancreas; li, liver; du, duodenum. Image published in: Beck CW and Slack JM (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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