Xenbase Image ID: 77336

Fig. 3. Xenopus tadpoles transgenic for transthyretin-nGFP or IFABP-GFP. (a-c) Transthyretin nGFP transgenics. (a,b) GFP reporter activity (green) in live tadpoles, yolk autofluorescence is yellow, anterior is to the right. (a) Four-day tadpole, ventral view, GFP is present in the liver (arrow) but absent from the rest of the gut, which autofluoresces yellow. (b) Six-day tadpole, ventral/left view to show GFP in the liver (li). The gall bladder (gb), to the left of the liver, fluoresces bright yellow at this stage, as does the small intestine (si). (c) In situ hybridisation to GFP mRNA in whole mount guts from transgenic tadpoles. (c) GFP is expressed exclusively in the liver (dark blue) at 5 days, anterior view. (d±f) Xenopus tadpoles transgenic for IFABP-GFP. (d,e) GFP reporter activity (green) in the coils of the small intestine of live 5-day (d) and 7-day (e) tadpoles, both ventral views, anterior to the left. Gall bladder (gb) autofluoresces yellow. (f) In situ hybridisation to GFP mRNA in 7 day whole mount gut. Expression of the reporter is seen in the small intestine (si, dark blue) but absent from the stomach (st), pancreas (pa), liver (li), gall bladder (gb) and colon (co). Abbreviations: gb, gall bladder; li, liver; si, small intestine; pa, pancreas; st, stomach; co, colon. Image published in: Beck CW and Slack JM (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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