Xenbase Image ID: 77343

Figure 4. Knockdown of miR-24a causes up-regulation of Caspase9 protein and is rescuable by its inhibition. (A) RT–PCR of caspase9 on stage 28 heads shows no change in mRNA levels between uninjected and miR-24a knockdown embryos. (B) Western blot for Caspase9 on stage 28 heads shows a significant increase in protein levels when miR-24a function is blocked (n = 3 experiments; error bars are SEM). (C) Coinjection of Caspase inhibitors to rescue the miR-24a knockdown phenotype. Only a pan-Caspase inhibitor (All) or an inhibitor specific to Caspase9 rescued the reduction in eye size caused by injection of 24aMO (n ≥ 47 for each treatment; error bars are SEM). Image published in: Walker JC and Harland RM (2009) Copyright © 2009. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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