Xenbase Image ID: 77344

Figure 5. miR-24a targets the 3′ UTR of caspase9 and apaf1. (A) The 3′UTRs of X. laevis caspase9 and apaf1 have two putative miR-24a-binding sites, as measured by RNAhybrid (Kruger and Rehmsmeier 2006). (B) The addition of miR-24a causes GFP reporters with miR-24a recognition elements in their 3′UTRs to be repressed. GFP reporters with the 3′UTR of caspase9 and apaf1 show repression by miR-24a. Mutating the putative miR-24a-binding sites in these constructs (mutCasp9 and mutApaf1) abolishes the repression (n = 4 experiments; error bars are SEM). (C) Embryos injected with 2 ng of miR-24a or miR-24amm or nothing (control) were cultured in 15 mM hydroxyurea to induce apoptosis at the onset of gastrulation. Only miR-24a was able to significantly repress or delay the onset of apoptosis (n = 3 experiments; error bars are SEM). Image published in: Walker JC and Harland RM (2009) Copyright © 2009. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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