XB-IMG-77379
Xenbase Image ID: 77379
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Fig. 1. A bilateral cluster of hyperpolarized cells appears in the anterior neural field before the formation of eye primordia. (A) CC2-DMPE staining (ii) showing the relative Vmem of cells in the indicated region (i) of a stage 18 Xenopus embryo. Arrowheads indicate the specific clusters of hyperpolarized cells in the anterior neural field. (B) Electrophysiological Vmem measurements of cells identified by CC2-DMPE staining versus their neighboring unstained cells. Readings were recorded from at least six embryos. Values are mean ± s.e.m. ***, P=0.0002. (C) CC2-DMPE staining (iii,iv) and, immediately thereafter, Rx1 in situ hybridization (v,vi) of the indicated regions (i,ii) of Xenopus embryos at stages 18 and 22. Red arrowheads mark specific clusters of hyperpolarized cells in the anterior neural field. Blue arrowheads indicate Rx1 expression in the same embryos. The bilateral hyperpolarization signal occurs before the formation of bilateral eye primordia. See also supplementary material Fig. S1, Table S1. Illustrations reproduced with permission from Nieuwkoop and Faber (Nieuwkoop and Faber, 1967). Image published in: Pai VP et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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