XB-IMG-77385
Xenbase Image ID: 77385
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Fig. S2. Knockdown of xMID has few specific effects. (A) Vegetal views of control-Mo-injected (Cont.-Mo, left) and xMID-Mo-injected (right) embryos at stage 11.5 (top) and 12.5 (bottom). Injection of xMID-Mo into the dorsal marginal zone of the four-cell embryo did not inhibit blastopore closure. (B) Animal cap elongation assay of control-Mo-injected (left) and xMID-Mo-injected (right) embryos. Injection of xMID-Mo did not inhibit animal cap elongation induced by activin mRNA. (C-E) Transverse sections through the neural plate of control-Mo-injected (left) and xMID-Mo-injected (right) embryos at stage 15.5, stained with antibodies against phospho-histone H3 (C, top), active caspase 3 (D, top) and Sox2 (E, top). Flag-β-globin mRNA (250 pg) was injected as a tracer, and stained with an anti-Flag antibody (middle). Knockdown of xMIDs did not have any obvious effects on mitosis, apoptosis or neural specification. (F) Transverse sections through the trunk ventral neural tube of control-Mo-injected (left) and xMID-Mo-injected (right) embryos at stage 27, stained with anti-acetylated tubulin antibody (Ac.-tubulin). mRNA encoding membrane-bound mRFP (125 pg) was injected as a tracer (magenta). Knockdown of xMIDs did not have any obvious effect on primary ciliogenesis in the neural tube. Dashed lines in C-F indicate the outlines of neural tissues. Scale bars: 50 in C-E; 10 in F. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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