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Fig. S3. In situ hybridization analysis of xMID morphants. (A-Z) Dorsal views of unilaterally injected morphants at stage 14-15; anterior is to the top. Morpholinos were injected into the right dorsal blastomere at the four-cell stage. Sox2 (A,B), NCAM (C,D) and N-cadherin (E,F) are pan-neural markers. Epidermal keratin (G,H) is an epidermal marker. HNF3β (FoxA2a) (I,J) and Pintallavis (FoxA4a) (K,L) are floor-plate markers. N-tubulin (M,N) is a marker for differentiated neurons. Shroom3 (O,P) is the essential regulator of apical constriction. Pax3 (Q,R) is a dorsal neural marker. Sonic hedgehog (Shh) (S,T), a key molecule for neural dorsoventral patterning, is expressed in the floor plate. Ptc2 (U,V) and Gli1 (W,X) are downstream targets in the Shh pathway. Gli3 (Y,Z) is a transcription factor mediating the Shh pathway. The expression patterns of all the markers in the xMID morphants were similar to those in control-Mo-injected embryos, except that the expression domains were wider than in the controls at given stages because of the delayed neural tube closure. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neuroectoderm cdh2.L cadherin-2, cdh2-a, cdh2-b, N-Cad, N-cadherin, ncad X. laevis Throughout NF stage 15 to NF stage 16 neural plate neuroectoderm foxa2.L HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 X. laevis Sometime during NF stage 15 to NF stage 16 neural tube floor plate neuroectoderm ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neuroectoderm krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 14 epidermis non-neural ectoderm foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Sometime during NF stage 15 to NF stage 16 neural tube floor plate neuroectoderm tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Sometime during NF stage 14 to NF stage 15 neural plate neuron Rohon-Beard neuron neuroectoderm pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 14 neural plate anterior neural ridge neuroectoderm neural plate border shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neural tube neural groove floor plate neuroectoderm shroom3.L apxl3, shrm, shroom, shroom-like, xshroom3 X. laevis Sometime during NF stage 14 to NF stage 15 neural plate neural tube anterior neural fold neural plate border neuroectoderm ptch2.L patched 2, patched-2, ptc-2, ptc2, ptch2-a, ptch2-b, xptc 2, xptc-2 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neural tube neural groove neuroectoderm gli1.S Gli-1, X-gli1 X. laevis Sometime during NF stage 15 to NF stage 17 neural plate neural tube neural plate border pre-chordal neural plate border chordal neural plate border neuroectoderm gli3.L XGli-3, Xgli3 X. laevis Sometime during NF stage 16 to NF stage 17 neural plate neural tube neural plate border pre-chordal neural plate border chordal neural plate border neuroectoderm anterior neural fold Image source: Published Larger Image Printer Friendly View

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