XB-IMG-77388
Xenbase Image ID: 77388
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Fig. S5. Tau partially rescues the neural tube defects in xMID morphants. (A) Dorsal views of the unilaterally injected xMID morphants, anterior to the top. Co-injection of tau mRNA (12.5 pg) reduced the distance between the neural folds. Dashed lines indicate the boundaries between neural and non-neural ectoderm. (B) The average distance between the neural folds of unilaterally injected xMID morphants was reduced by tau mRNA (6.25, 12.5 pg). The number of embryos examined is indicated above each bar. (C-F) Transverse sections through the neural plate of unilaterally injected xMID morphants with (D,F) or without (C,E) tau (12.5 pg), stained with phalloidin (C,D) or anti-α-tubulin antibody (E,F). EGFP mRNA (50 pg) was co-injected as a tracer (C′,D′). Schematic illustrations of morphant cells (outlined in pink) in C,D are shown. (G-L) Quantification of cell morphological features in the superficial layer. Control-Mo (Cont.-Mo), n=41 cells, 7 embryos; Control-Mo + tau, n=43 cells, 9 embryos; xMID-Mo, n=86 cells, 13 embryos; xMID-Mo + tau, n=65 cells, 9 embryos. *P<0.01, **P<0.005, ***P<0.001, compared with xMID-Mo. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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