XB-IMG-77453
Xenbase Image ID: 77453
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Fig. S2. Cell division is dispensable for Xenopus NTC. (A-E) Inhibition of cell division by HUA treatment. The cell division of control (A,B) and HUA-treated (C,D) embryos was analyzed by immunostaining using an antibody for phospho-histone H3 (pHH3), a mitotic index. The ratio of pHH3-positive nuclei to total nuclei in HUA-treated embryos was significantly lower than that of control embryos (E). n=24 (stage 14), 24 (stage 16) and 15 (stage 20) for control embryos; n=26 (stage 14), 28 (stage 16) and 18 (stage 20) for HUA-treated embryos. Data are mean.e.m. (F,G) Hydroxyurea and aphidicolin (HUA) treatment during NTC. Serial images of control (F) and HUA-treated (G) embryos taken from time-lapse movies are shown. Embryos under both conditions closed their NT at the same speed. (H,I) Transverse sections of control (H) and HUA-treated (I) embryos fixed after the NT of the control was closed (stage 22). Scale bars: 100 in A,C; 50 in H,J. Image published in: Morita H et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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