Xenbase Image ID: 77456

Fig. S5. Integrin-β1 expression and efficiency of its knockdown. (A-D) Localization of integrin-β1 and fibronectin (FN). Integrin-β1 (A,B) and FN (C,D) were highly localized to the border between ectoderm and mesoderm with high density (arrowheads). (E) Depletion of exogenous integrin-β1 by Itgβ1-MO. Venus (vns)-tagged integrin-β1 was depleted by the co-injection of Itgβ1-MO, whereas a rescue construct of integrin-β1 (integrin-β1-res-vns) was not affected by the MO. (F) Depletion of endogenous integrin-β1 by Itgβ1-MO. Non-neural ectoderm of the control and Itgβ1-MO-injected embryos were subjected to western blot. The endogenous integrin-β1 protein was significantly reduced. (G-K) Effect of depletion of integrin-β1 on cell death. The control (G,H) and Itgβ1-MO-injected (I,J) embryos were immunostained with an anti-active caspase-3 antibody, a marker for the early stages of apoptosis. Arrowheads indicate active caspase-3-positive nuclei. The ratio of active caspase-3-positive nuclei to total nuclei indicates that injection of Itgβ1-MO did not significantly affect cell death (K). n=12 for control embryos and n=11 for Itgβ1-MO-injected embryos; data are mean.e.m.; n.s., not significant, t-test. (L) Relative thickness of the non-neural superficial cells during neurulation. The cell height along the apical-basal axis was measured in the superficial layer of the lateral non-neural ectoderm in control and Itgβ1-MO-injected embryos. n=8 for control embryos and n=6 for Itgβ1-MO-injected embryos; data are mean.e.m.; n.s., not significant, t-test. (M) Number of cells that intercalate radially towards the surface of embryo during neurulation. Radially intercalating cells were counted in DSLM images of wil-type embryos. n=3; data are mean.e.m. Scale bars: 200 in A,C,G,I; 100 in B; 50 in D. Image published in: Morita H et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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