XB-IMG-77490
Xenbase Image ID: 77490
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Figure 4. Missplicing of cdh1 underlies the cell adherence defect in fus morphants. (A) RT–PCR of full-length cadherin transcripts showing significant and specific reduction of cdh1 in fus morphants, whereas bcad and ccad levels are normal. odc was used as RT–PCR control. (B) Intron retention of cdh1 in fus morphants causes a decrease in properly spliced transcripts. RT–PCR using primers covering the first five exons of cdh1 detects two aberrant transcripts retaining intron 3 (cdh1-in3ret) or introns 3 and 4 (cdh1-in3+4ret), respectively. (C–J) Restoration of cell adherence by cdh1 and fus mRNA in fusMO-injected animal caps. (C) Control animal cap forms an epidermal ball (24 of 24 caps had epithelial integrity). (D) The fusMO-injected animal cap completely dissociates into individual cells (zero of 22 showed integrity). (E) Epithelial integrity in animal cap coinjected with fusMO and cdh1 mRNA (14 of 19). (F) Epithelial integrity in animal cap coinjected with fusMO and MO-resistant fus mRNA (13 of 16). (G–J) Red fluorescence emission from coinjected mCherry mRNA used as tracer in E and F. Image published in: Dichmann DS and Harland RM (2012) Copyright © 2012. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |