Xenbase Image ID: 77491

Figure 5. Pervasive intron retention in fus morphants detected by RNA-seq. In all panels, the top yellow and gray track shows the JGI v7.1 gene models for reference. The black tracks show the control read profile, and the red tracks show read profile for fus morphants. (A) Two splice forms, fgfr2b and fgfr2c, are detected in control lanes at stage 10, whereas only fgfr2b is detected in fus morphants. The alternatively spliced region is boxed in blue. At stage 12, the control read distribution is similar to that at stage 10, whereas the fusMO lane shows a complete flattening of the transcript profile, indicating that all introns are retained. (B) Normal exon profile of cdh1 in control lane at stage 10; dramatic intron retention is apparent in fus morphants. (C) The bcad transcript is normal in fus morphants at stage 10. Image published in: Dichmann DS and Harland RM (2012) Copyright © 2012. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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