Xenbase Image ID: 77511

Fig. 4. XSeb4R stabilizes maternal Sox3 mRNAs in ectodermal explants. Capped-XSeb4R mRNA and XSeb4R-MO were injected alone or in combination with α-amanitin into the four animal blastomeres of 4/8-cell stage embryos. The latter were cultured together with control uninjected embryos to blastula stage. Cap explants were dissected and cultured (A) at different time points as indicated and an average of 50 caps was collected per batch for mRNA analysis. The real-time RT-PCR results reveal significant increase of Sox3 (B) and VegT (C) mRNA in XSeb4R-overexpressing caps. Note increased levels of Sox3 but not VegT mRNA in XSeb4R-MO samples (B and C, yellow bars). This increase of Sox3 mRNA levels was lost upon α-amanitin treatment (D). Alpha-amanitin treated cap explants were compared to naive or XSeb4R-overexpressing cap samples (E–F). Note the unchange levels of Sox3 mRNA (E), unlike Epi. K. (F), in treated and untreated cap explants. In XSeb4R-injected caps, the levels of Sox3 mRNA, but not Epi. K., reamined significantly high compared to the control untreated naive caps (E and F). The RNA quantification was done in duplicate or triplicate and the standard deviation is added to the histogrammes. Data are expressed relative to GAPDH mRNA. Note a use of different scales in graphics from panels B to F. Image published in: Bentaya S et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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