Xenbase Image ID: 77514

Fig. 1. Otx2 and Gbx2 form a boundary within the PPR. (A) Expression of Eya2 marks the PPR (bracket). (B)–(D) Double ISH for Otx2 (red) and Gbx2 (blue) at HH7. Lines indicate the level of sections shown in (C) and (D). (E)–(J) Parasagittal sections of stage HH5 (E)–(G) and HH7 (H)–(J) chick embryos after Gbx2 ISH (black (E), (F), (H), (I)) and Otx2 immunostaining (green; (F), (G), (I), (J)); DAPI labels nuclei ((G), (J); magenta). Arrows in (H)–(J) indicate anterior limit of Gbx2. (K) and (L) Double ISH in Xenopus for Otx2 ((K), (L); turquoise) and Gbx2 ((L); blue) at stage 12, dorsal to right anterior to the top. Red brackets indicate overlapping expression. (M) Diagram summarising expression of Otx2 and Gbx2 at stage 12 in Xenopus; red bracket: PPR. (N) Eya1 at stage 13 labels the PPR (bracket). (O) and (P) Double ISH for Otx2 ((O), (P); turquoise) and Gbx2 ((P); blue) at stage 13, dorsal to the right. Black brackets indicate the PPR. (Q) Diagram summarising neural plate and PPR expression of Otx2 and Gbx2 at stage 13 in Xenopus Image published in: Steventon B et al. (2012) © 2012 Elsevier Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 12 involuted dorsal mesoderm neural plate otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 13 neural plate gbx2.2.L XGBX-2 X. laevis Throughout NF stage 12 dorso-lateral ectoderm gbx2.2.L XGBX-2 X. laevis Throughout NF stage 13 ectoderm dorso-lateral eya1.L eyes absent-1, XEya1 X. laevis Throughout NF stage 13 preplacodal ectoderm Image source: Published Larger Image Printer Friendly View

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