Xenbase Image ID: 77519

Fig. 6. Activation of Otx2 target genes is required at early and late stages of lens placode formation. (A), (B) Otx2-EnR inhibits Pax6 in the lens at stage 17 (50%, n=26). This is rescued by co-injection of Otx2 ((B); inhibition reduced to 15%, n=20). (C)–(F) Activation of Otx2-EnR-GR at stage 10 reduces lens-specific Pax6 at stage 18 ((D) 52%, n=25); without DEX Pax6 expression is normal ((C) 0% affected, n=23). FoxE3 at stage 25 is normal without DEX ((E) 0% affected, n=30), while addition of DEX at stage 18 leads to reduction ((F) 64%, n=22). (G) Otx2-E1A activates Otx2 targets but does not affect lens Pax6 (0% affected, n=25). All embryos were injected into the A3 blastomere at the 32-cell stage and are shown in frontal view with dorsal to the top. High magnifications of the lens region are shown below each panel; dotted lines demarcate placodal Pax6. Turquoise staining reveals the lineage tracer FDX. Image published in: Steventon B et al. (2012) © 2012 Elsevier Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 17 lens optic field pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 17 lens optic field foxe3.L foxe1, FoxE4, lens1, Xlens1 X. laevis Throughout NF stage 25 optic vesicle Image source: Published Larger Image Printer Friendly View

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