Xenbase Image ID: 77520

Fig. 7. Activation of Otx2 target genes is required at early and late stages of olfactory placode formation. (A), (B) Otx2-EnR inhibits the olfactory placode marker Dmrt4 at stage 21 (59%, n=29). This is rescued by co-injection of Otx2 ((B) inhibition reduced to 12%, n=33). (C–F) Otx2-EnR-GR injections: in the absence of DEX Dmrt4 expression is normal ((C) 5% affected, n=20); when DEX is added at stage 10 Dmrt4 expression is lost at stage 18 ((D) 59%, n=44). At stage 25 Dmrt4 is normal in absence of DEX ((E) 8% affected, n=26), while activation at stage 18 strongly reduces Dmrt4 ((F); 63%, n=24). (G) Otx2-E1A has no effect on Dmrt4 (0% affected, n=14). (H)–(I) Otx2 mRNA reduces Pax2 ((H) 72%, n=25), but does not change Eya1 ((I) 0% affected, n=44). All embryos were injected into the A3 blastomere at the 32-cell stage and are shown in frontal view with dorsal to the top. High magnifications of the olfactory region are shown in small panels; dotted lines demarcate placodal Dmrt4. Turquoise staining reveals the lineage tracer FDX. Image published in: Steventon B et al. (2012) © 2012 Elsevier Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue dmrta1.S dmrt4, XDmrt4 X. laevis Throughout NF stage 21 olfactory region dmrta1.S dmrt4, XDmrt4 X. laevis Throughout NF stage 18 olfactory region neural plate dmrta1.S dmrt4, XDmrt4 X. laevis Throughout NF stage 25 olfactory placode pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 18 presumptive midbrain-hindbrain boundary optic field Image source: Published Larger Image Printer Friendly View

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