Xenbase Image ID: 77623

Fig. 2. Xenopus Zic3 morphants exhibit defective gastrulation and convergent extension morphogenesis. (A) Normal control (CTRL) MO embryos at gastrula stage. (B) Sibling Zic3 morphants at stage 12.25 with delayed and abnormal blastopore closure. (C) The percentage of control morpholino and Zic3 morphant embryos that showed blastopore closure defects. (D–E, G–H) Whole-mount in situ hybridization (WISH) of brachyury at stage 13. (D) The blastopore area is outlined by the dashed line in control morpholino (D) and Zic3 morphant (E) embryos. (F) Average blastopore area in control and Zic3 morphant embryos. (G) Notochord length (L) and width (W) are designated by white lines in control morpholino embryos (G) and Zic3 morphants (H), which exhibited a wider, shorter notochord. (I) Average notochord length-to-width ratio in control morpholino and Zic3 morphant embryos. (J) Anterior–posterior (A–P) axis measurement (white line) of control morphant (J) and Zic3 morphant (K) embryos at the tailbud stage. (L) Average A–P axis lengths in control and Zic3 morphants. Error bars represent SEM. *p < 0.05; **p < 0.01 by t-test. Image published in: Cast AE et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 13 circumblastoporal collar notochord tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 33 and 34 notochord head region eye otic vesicle Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + zic3 MO + NF12.5 (morphology) fig.2.b Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore abnormally increased area of blastopore Xla Wt + zic3 MO + NF13 (morphology) fig.2.e Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore abnormally increased area of blastopore Xla Wt + zic3 MO + NF13 (in situ hybridization) fig.2.h Anatomical Phenotype abnormal development of notochord decreased length of notochord Xla Wt + zic3 MO + NF12.5 (morphology) fig.2.k Anatomical Phenotype decreased length of anterior-posterior axis Larger Image Printer Friendly View

Return to previous page