Xenbase Image ID: 77655

Fig. 3. Rescue of Zic3-attenuated notochord progenitor marker expression by co-injection of Siamois RNA. (A) Xnot expression in early gastrula. Vegetal views of the embryos with dorsal at the top. The embryos were injected with the RNAs indicated below (1000 pg each). Xnot expression was detected in the dorsal area adjacent to the yolk plug (purple signal) in the uninjected (uninj) embryo and the β-galactosidase (β-gal)-injected embryo, but disappeared in the Zic3 RNA (Zic3)-injected embryo. β-gal, Zic3, Bra, Sia and Nr3 mean the injected RNAs of β-galactosidase, Zic3, Bra, Siamois and Nr3, respectively. (B) Percentages of embryos with Xnot expression. Co-injection of Siamois fully restored normal Xnot expression. *P < 0.01; **P < 0.001; n.s., P > 0.05 in comparison to Zic3 alone injection in χ2 test. More than 50 embryos were analyzed in each group. Image published in: Fujimi TJ et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue not.S flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Throughout NF stage 10.5 dorsal marginal zone notochord upper blastopore lip non-involuting marginal zone axial mesoderm Image source: Published Larger Image Printer Friendly View

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