XB-IMG-77658
Xenbase Image ID: 77658
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Fig. 6. β-catenin nuclear localization is impaired by Zic3 expression in gastrula embryos. (A) Representative images for β-catenin immunofluorescence staining. Neighboring sections were stained with anti-β-catenin and anti-β-galactosidase (β-gal) antibodies. In each image, the animal pole is at the top and the dorsal side is to the right. Upper images show the β-catenin signal (nuclear, green; non-nuclear, blue) and DAPI signal (nuclear, red), and lower images show the β-galactosidase signal (gray) of the injected area. The yellow indicates the overlapping of nuclear β-catenin and DAPI signals. The insets are low magnifications in which the boxed regions indicate the stained regions. (B) The graph shows percentages of the β-catenin/DAPI double-positive cell nuclei area among the total DAPI-stained cell nuclei in the organizer region. Nuclear transported β-catenin was significantly lower in Zic3-injected embryos. **P < 0.01, Kruskal–Wallis test followed by Steel–Dwass post hoc test. Error bars, standard error of the mean. (C) Immunoblotting analysis for β-catenin. Embryos were injected with the β-catenin or (β-catenin and Zic3) RNA. Immunoblotting of the embryo tissue lysate using an antibody against the anti-β-catenin C-terminus (β-cat.) or an antibody against the Flag-tag located on the N-terminus of β-catenin (Flag). Zic3 RNA co-injection enhanced the degradation of β-catenin. Image published in: Fujimi TJ et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
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