Xenbase Image ID: 77659

Fig. 7. Zic3 can physically interact with Tcf1 and XIC. Immunoprecipitation experiments indicating the physical interaction among Zic3, Tcf1, and XIC. Epitope-tagged Xenopus Tcf1, Zic3, and XIC were expressed in COS7 cells in the indicated combinations. Following immunoprecipitation (IP) with an anti-HA-tag antibody (IP_HA), the co-precipitated proteins were detected with an anti-Myc-tag antibody. The cell lysates (input) used for the immunoprecipitation experiments were also subjected to an immunoblot analysis (input_IB) using the antibodies against the epitope tags (HA, Myc, and Flag) and actin. Both the Tcf1 (left) and Zic3 (right) pull-down experiments suggested that Zic3 and Tcf1 physically interacted, and that this interaction was enhanced by XIC. XIC was co-precipitated with both Zic3 and Tcf1. Image published in: Fujimi TJ et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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