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Suppl. Fig. 1. Spatial expression pattern of FMR1 and FXR1. A. WMISH detecting FMR1. At stage 15 a weak FMR1 expression can be detected in anterior neural tissue (white arrowhead). At stage 20, FMR1 transcripts can be visualized in the neural tube (red arrow), the migrating cranial NC cells (white arrow) and the eye anlage (black arrow). Expression in the eye and the NC is maintained at later stages 25, 28, and 33. FMR1 is also expressed in the otic vesicle (stage 25, green arrow) and the pronephric nephrostomes (stage 33, green arrowheads). At stage 33 FMR1 is expressed in the lens and the ciliary marginal zone of the eyes (left vibratome section on the level of the white dotted line) and parts of the pharyngeal arches (right vibratome section on the level of the yellow dotted line, white arrow). B. Spatial expression pattern of FXR1. In all examined stages, FXR1 transcripts can be detected in the somites (e.g. stage 28 red arrowhead) and at stage 40 in the migrating hypaxial muscle cells (red arrowhead). At stage 20, FXR1 is additionally expressed in the anterior neural tissue (white arrowhead).
