Xenbase Image ID: 77768

Fig. 1. XSIP1 MO inhibited neural development. (A) Western blot analysis of myc-tagged proteins. SDS-PAGE was performed with protein prepared from embryos injected into the blastomere of the two cell stage with 1 ng of XSIP1-MT mRNA or rXSIP1-MT mRNA either with or without 40 ng of XSIP1 MO. α-Actin served as an internal control. (B) XSIP1 MO specifically inhibited neural differentiation induced by XSIP1 mRNA. All embryos were injected with either 1 ng of XSIP1 or rXSIP1 mRNA alone or with 40 ng of XSIP1 MO. Animal caps were dissected at stage 9 and cultured. When sibling embryos reached stage 28, total RNA from 30 explants was extracted and some marker genes were examined by RT-PCR. (C–E) Embryos injected with XSIP1 MO showed defects in neural and eye development. (C) Uninjected embryo. (D) Forty nanogram of XSIP1 MO was injected into the dorsal-animal pole of the eight-cell-stage embryo. (E) Another embryo similarly injected with 40 ng of XSIP1 MO and 50 pg of linearized pCS2-rXSIP1. (F–I) Transverse sections of control MO (F,G) and XSIP1 MO (H,I) injected embryos. Scale bars indicate 100 μm. Image published in: Nitta KR et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page