Xenbase Image ID: 77769

Fig. 2. Injection of XSIP1 MO resulted in the down-regulation of terminal neural marker genes. Whole-mount in situ hybridization analysis of N-tubulin (A,B) and N-CAM (C,D) performed on stage 28 embryos. Embryos were injected with 40 ng of either control MO or XSIP1 MO into one dorsal-animal blastomere of the eight-cell stage, and co-injected with 250 pg of nuclear-localized lacZ as a lineage tracer (red stained). (A,C) Control MO-injected embryo. Injected area (red) and marker expression (blue) overlapped. (B, D) XSIP1 MO-injected embryo. Neural markers, N-tubulin and N-CAM were not expressed in the injected area. Inset; Dorsal view of the same embryos. Image published in: Nitta KR et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 28 central nervous system brain spinal cord forebrain midbrain hindbrain profundal placode trigeminal placode facial epibranchial placode ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 28 central nervous system brain spinal cord eye forebrain midbrain hindbrain Image source: Published Larger Image Printer Friendly View

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