Xenbase Image ID: 77774

Fig. 7. Injection of SoxD MO into Xenopus embryos resulted in a neural defect phenotype. (A) Western blot analysis of HA-tagged proteins. SDS-PAGE was performed with protein prepared from embryos injected with 1 ng SoxD-HA(UTR+) or SoxD-HA(UTR−) mRNA either with or without 40 ng SoxD MO and cultured until stage 9. α-Actin served as an internal control. (B–D) Phenotype of embryos injected with 20 ng SoxD MO. (B) Uninjected embryo. (C) SoxD MO was injected into dorsal-animal blastomeres of eight-cell-stage embryos. (D) Linerized pCS2-SoxD-ORF (100 pg) was co-injected with SoxD MO into dorsal-animal blastomeres of eight-cell-stage embryos. (E–J) Whole-mount in situ hybridization analysis of terminal differentiation markers N-tubulin (E–H) and N-CAM (I,J). Embryos were injected with 40 ng SoxD MO into dorsal-animal blastomeres of eight-cell-stage embryos (F,H,J) and cultured until stage 15 (E,F) or 25 (G–J). (E,F) Dorsal view. Image published in: Nitta KR et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 15 neuron Rohon-Beard neuron tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 25 spinal cord brain forebrain midbrain hindbrain central nervous system ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 25 eye central nervous system brain spinal cord forebrain midbrain hindbrain Image source: Published Larger Image Printer Friendly View

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